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TITLE Development of Equipment of Measuring the
Microorganism Activity

(Joint Program to Promote Technological Development with the Private Sectors)

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The bioremediation of the hazardous oil waste has been recently taken on new significance as an effective technology. We are developing the technology for evaluating the activity of the microbiology used for bioremediation of oil pollution.

Our currently developed "Equipment of Measuring the Microorganism Activity" enables to measure the pH distribution generated by the cell metabolite.

In order to develop this equipment, we have improved the both spatial resolution and sensitivity of novel two-dimensional pH imaging technology. As the results, we have attained 5 micron spatial resolution and 0.01 pH sensitivity. Using a prototype for this technique, microscopic pH distribution generated by a metabolism of microorganism could be imaged and numerically evaluated.