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  3. Takeshi Kubota

Takeshi Kubota

Job Title

Senior Researcher

Areas of speciality


Research areas

Fermentation production of aromatic compounds

Professional affiliations

The Japan Society for Bioscience, Biotechnology, and Agrochemistry

Major accomplishment

Kubota T, Watanabe A, Suda M, Kogure T, Hiraga K and Inui M. Production of para-aminobenzoate by genetically engineered Corynebacterium glutamicum and non-biological formation of an N-glucosyl byproduct. Metab. Eng. 38:322-330. 2016.

Kubota T, Tanaka Y, Takemoto N, Hiraga K, Yukawa H and Inui M. Identification and expression analysis of a gene encoding a shikimate transporter of Corynebacterium glutamicum. Microbiology 161:254-263. 2015.

Kubota T, Tanaka Y, Takemoto N, Watanabe A, Hiraga K, Inui M and Yukawa H. Chorismate-dependent transcriptional regulation of quinate/shikimate utilization genes by LysR-type transcriptional regulator QsuR in Corynebacterium glutamicum: carbon flow control at metabolic branch point. Mol. Microbiol. 92: 356-368. 2014.

Kubota T, Tanaka Y, Hiraga K, Inui M, Yukawa H. Characterization of Shikimate Dehydrogenase Homologues of Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 97: 8139-8149. 2013.

Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A. Cy5-conjugated Hybridization-sensitive Fluorescent Oligonucleotides for Ratiometric Analysis of Nuclear poly(A) + RNA. Bioconjug. Chem. 22: 1625-1630. 2011.

Ikeda S, Kubota T, Wang DO, Yanagisawa H, Umemoto T, Okamoto A. Design and Synthesis of Caged Fluorescent Nucleotides and Application to Live-cell RNA Imaging. Chembiochem. 12:2871-2880. 2011.

Ikeda S, Kubota T, Wang DO, Yanagisawa H, Yuki M, Okamoto A. Emission Control by Binary Energy Transfer Processes on Oligouridine. Org. Biomol. Chem. 9: 6598-6603. 2011.

Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A. Sets of RNA Repeated Tags and Hybridization-Sensitive Fluorescent Probes for Distinct Images of RNA in a Living Cell. PLoS ONE. 5: e13003. 2010.

Ikeda S, Kubota T, Yuki M, Yanagisawa H, Tsuruma S, Okamoto A. Hybridization-sensitive fluorescent DNA probe with self-avoidance ability. Org. Biomol. Chem. 8: 546-551. 2010.

Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A. Hybridization-Sensitive Fluorescent Probe for Long-Time Monitoring of Intracellular RNA. Bioconjug. Chem. 20: 1256-1261. 2009.

Ikeda S, Kubota T, Yuki M, Okamoto A. Exciton-Controlled Hybridization-sensitive Fluorescent Probes: New Approach to Multicolor Detection of Nucleic Acids. Angew. Chem. Int. Ed. 48: 6480-6484. 2009.

Kubota T, Ikeda S, Okamoto A. Doubly Thiazole Orange-Labeled DNA for Live Cell RNA Imaging. Bull. Chem. Soc. Jpn. 82: 110-117. 2009.

Ikeda S, Kubota T, Kino K, Okamoto A. Sequence Dependence of Fluorescence Emission and Quenching of Doubly Thiazole Orange-Labeled DNA: Effective Design of a Hybridization-Sensitive Probe. Bioconjug. Chem. 19: 1719-1725. 2008.

Kubota T, Shindo Y, Tokuno K, Komatsu H, Ogawa H, Kudo S, Kitamura Y, Suzuki K, Oka K. Mitochondria are Intracellular Magnesium Stores: Investigation by Simultaneous Fluorescent Imagings in PC12 Cells. Biochim Biophys Acta-Molecular Cell Research. 1744: 19-28. 2005.

Takatsuna H, Asagiri M, Kubota T, Oka K, Osada T, Sugiyama C, Saito H, Aoki K, Ohya K, Takayanagi H, Umezawa K. Inhibition of RANKL-induced Osteoclastogenesis by (-)-DHMEQ, a Novel NF-kappaB Inhibitor, through Downregulation of NFATc1. J. Bone. Miner. Res. 20: 653-662. 2005.

Komatsu H, Miki T, Citterio D, Kubota T, Shindo Y, Kitamura Y, Oka K, Suzuki K. Single Molecular Multianalyte (Ca2+, Mg2+) Fluorescent Probe and Applications to Bioimaging. J Am Chem Soc. 127: 10798-10799. 2005.

Komatsu H, Iwasawa N, Citterio D, Suzuki Y, Kubota T, Tokuno K, Kitamura Y, Oka K, Suzuki K. Design and Synthesis of Highly-sensitive and -selective Fluorescein-Derived Magnesium Fluorescent Probes and Application to Intracellular 3D-Mg2+ Imaging. J. Am. Chem. Soc. 126: 16353-16360. 2004.

Kubota T, Tokuno K, Nakagawa J, Kitamura Y, Ogawa H, Suzuki Y, Suzuki K, Oka K. Na+/Mg2+ Transporter Acts as a Mg2+ Buffering Mechanism in PC12 cells. Biochem. Biophys. Res. Commun. 303: 332-336. 2003.

Suzuki Y, Komatsu H, Ikeda T, Saito N, Araki S, Citterio D, Hisamoto H, Kitamura Y, Kubota T, Nakagawa J, Oka K, Suzuki K. Design and Synthesis of Mg2+-selective Fluoroionophores Based on a Coumarin Derivative and Application for Mg2+ Measurement in a Living Cell. Anal. Chem. 74: 1423-1428. 2002.

Ohtsuka Y, Kubota T, Ikeno T, Nagata T, Yamada T. Convenient Preparation of Optically Pure 1, 3-Diaryl-1,3-propanediols. Synlett. 535-537. 2000.

Kubota T, Oka K. Simultaneous Fluorescent Imaging of Mg2+ and Ca2+. Clinical Calcium. 14: 1219-1222. 2004.