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  3. 久保田 健

久保田 健

役職

主任研究員

専門分野

生物物理学

担当研究分野

芳香族化合物のグリーン生産プロセス開発

学歴

慶應義塾大学大学院 理工学研究科 基礎理工学専攻 博士(理学)

職歴

2005-2007 株式会社ニコン インストルメンツカンパニー
2007-2011 独立行政法人 理化学研究所 基幹研究所
2011- 現職

所属学会

日本農芸化学会

主要業績

Kubota T, Watanabe A, Suda M, Kogure T, Hiraga K and Inui M. Production of para-aminobenzoate by genetically engineered Corynebacterium glutamicum and non-biological formation of an N-glucosyl byproduct. Metab. Eng. 38: 322-330. 2016.

Kubota T, Tanaka Y, Takemoto N, Hiraga K, Yukawa H and Inui M. Identification and expression analysis of a gene encoding a shikimate transporter of Corynebacterium glutamicum. Microbiology 161: 254-263. 2015.

Kubota T, Tanaka Y, Takemoto N, Watanabe A, Hiraga K, Inui M and Yukawa H. Chorismate-dependent transcriptional regulation of quinate/shikimate utilization genes by LysR-type transcriptional regulator QsuR in Corynebacterium glutamicum: carbon flow control at metabolic branch point. Mol. Microbiol. 92: 356-368. 2014.

Kubota T, Tanaka Y, Hiraga K, Inui M, Yukawa H. Characterization of Shikimate Dehydrogenase Homologues of Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 97: 8139-8149. 2013.

Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A. Cy5-conjugated Hybridization-sensitive Fluorescent Oligonucleotides for Ratiometric Analysis of Nuclear poly(A) + RNA. Bioconjug. Chem. 22: 1625-1630. 2011.

Ikeda S, Kubota T, Wang DO, Yanagisawa H, Umemoto T, Okamoto A. Design and Synthesis of Caged Fluorescent Nucleotides and Application to Live-cell RNA Imaging. Chembiochem. 12:2871-2880. 2011.

Ikeda S, Kubota T, Wang DO, Yanagisawa H, Yuki M, Okamoto A. Emission Control by Binary Energy Transfer Processes on Oligouridine. Org. Biomol. Chem. 9: 6598-6603. 2011.

Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A. Sets of RNA Repeated Tags and Hybridization-Sensitive Fluorescent Probes for Distinct Images of RNA in a Living Cell. PLoS ONE. 5: e13003. 2010.

Ikeda S, Kubota T, Yuki M, Yanagisawa H, Tsuruma S, Okamoto A. Hybridization-sensitive fluorescent DNA probe with self-avoidance ability. Org. Biomol. Chem. 8: 546-551. 2010.

Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A. Hybridization-Sensitive Fluorescent Probe for Long-Time Monitoring of Intracellular RNA. Bioconjug. Chem. 20: 1256-1261. 2009.

Ikeda S, Kubota T, Yuki M, Okamoto A. Exciton-Controlled Hybridization-sensitive Fluorescent Probes: New Approach to Multicolor Detection of Nucleic Acids. Angew. Chem. Int. Ed. 48: 6480-6484. 2009.

Kubota T, Ikeda S, Okamoto A. Doubly Thiazole Orange-Labeled DNA for Live Cell RNA Imaging. Bull. Chem. Soc. Jpn. 82: 110-117. 2009.

Ikeda S, Kubota T, Kino K, Okamoto A. Sequence Dependence of Fluorescence Emission and Quenching of Doubly Thiazole Orange-Labeled DNA: Effective Design of a Hybridization-Sensitive Probe. Bioconjug. Chem. 19: 1719-1725. 2008.

Kubota T, Shindo Y, Tokuno K, Komatsu H, Ogawa H, Kudo S, Kitamura Y, Suzuki K, Oka K. Mitochondria are Intracellular Magnesium Stores: Investigation by Simultaneous Fluorescent Imagings in PC12 Cells. Biochim Biophys Acta-Molecular Cell Research. 1744: 19-28. 2005.

Takatsuna H, Asagiri M, Kubota T, Oka K, Osada T, Sugiyama C, Saito H, Aoki K, Ohya K, Takayanagi H, Umezawa K. Inhibition of RANKL-induced Osteoclastogenesis by (-)-DHMEQ, a Novel NF-kappaB Inhibitor, through Downregulation of NFATc1. J. Bone. Miner. Res. 20: 653-662. 2005.

Komatsu H, Miki T, Citterio D, Kubota T, Shindo Y, Kitamura Y, Oka K, Suzuki K. Single Molecular Multianalyte (Ca2+, Mg2+) Fluorescent Probe and Applications to Bioimaging. J Am Chem Soc. 127: 10798-10799. 2005.

Komatsu H, Iwasawa N, Citterio D, Suzuki Y, Kubota T, Tokuno K, Kitamura Y, Oka K, Suzuki K. Design and Synthesis of Highly-sensitive and -selective Fluorescein-Derived Magnesium Fluorescent Probes and Application to Intracellular 3D-Mg2+ Imaging. J. Am. Chem. Soc. 126: 16353-16360. 2004.

Kubota T, Tokuno K, Nakagawa J, Kitamura Y, Ogawa H, Suzuki Y, Suzuki K, Oka K. Na+/Mg2+ Transporter Acts as a Mg2+ Buffering Mechanism in PC12 cells. Biochem. Biophys. Res. Commun. 303: 332-336. 2003.

Suzuki Y, Komatsu H, Ikeda T, Saito N, Araki S, Citterio D, Hisamoto H, Kitamura Y, Kubota T, Nakagawa J, Oka K, Suzuki K. Design and Synthesis of Mg2+-selective Fluoroionophores Based on a Coumarin Derivative and Application for Mg2+ Measurement in a Living Cell. Anal. Chem. 74: 1423-1428. 2002.

Ohtsuka Y, Kubota T, Ikeno T, Nagata T, Yamada T. Convenient Preparation of Optically Pure 1, 3-Diaryl-1,3-propanediols. Synlett. 535-537. 2000.

久保田健, 岡本晃充. 「RNAの細胞内局在化の蛍光イメージング法」. 組織細胞化学2010 111-121. 2010.

久保田健, 池田修司, 岡本晃充. 「光化学的に設計された新規蛍光核酸によるDNA/RNAイメージング」. 生物物理 49: 310-313. 2009.

Kubota T, Oka K. Simultaneous Fluorescent Imaging of Mg2+ and Ca2+. Clinical Calcium. 14: 1219-1222. 2004.

特開2011-173891
発明の名称:「モノヌクレオシドまたはモノヌクレオチドから誘導される構造を有する化合物、核酸、標識物質、核酸検出方法およびキット」発明者:岡本 晃充,池田 修司,久保田 健. 出願人:独立行政法人理化学研究所.

科学研究費助成事業・若手研究(B)・研究課題番号16K18305・研究代表者 2016-2017年度 「低コストかつ高効率な物質生産を可能にする迅速な代謝経路切り替えシステムの構築」

科学研究費助成事業・若手研究(B)・研究課題番号21750182・研究代表者 2009-2010年度 「生細胞内RNAの効率的時空間解析のための励起子制御型汎用的タグシステム」

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