|Identification of a HAD superfamily phosphatase, HdpA, involved in 1,3-dihydroxyacetone production during sugar catabolism in Corynebacterium glutamicum.
FEBS Lett. 586: 4228-4232. 2012.
T. Jojima, T. Igari, W. Gunji, M. Suda, M. Inui and H. Yukawa.
|Corynebacterium glutamicum produces 1,3-dihydroxyacetone (DHA) as metabolite of sugar catabolism
but the responsible enzyme is yet to be identified. Using a transposon
mutant library, the gene hdpA (cgR_2128) was shown to encode a haloacid dehalogenase superfamily member that catalyzes dephosphorylation of dihydroxyacetone phosphate to produce DHA. Inactivation of hdpA led to a drastic decrease in DHA production from each of glucose, fructose,
and sucrose, indicating that HdpA is the main enzyme responsible for DHA
production from sugars in C. glutamicum. Confirmation of DHA production via dihydroxyacetone phosphatase finally
confirms a long-speculated route through which bacteria produce DHA.