|Postgenomic approaches to using corynebacteria as biocatalysts.
Annu Rev Microbiol. 66: 521-550. 2012.
A.A. Vertès, M. Inui and H. Yukawa.
|Corynebacterium glutamicum exhibits numerous ideal intrinsic attributes as a factory of primary and
secondary metabolites. The versatile capabilities of this organism have
long been implemented at the industrial scale to produce an array of amino
acids at high yields and conversion rates, thereby enabling the development
of an entire industry. The postgenomic era provides a new technological
platform not only to further optimize the intrinsic attributes of C. glutamicum whole cells as biocatalysts, but also to dramatically expand the product
portfolio that can be manufactured by this organism, from amino acids to
commodity chemicals. This review addresses the methods and strain optimization
strategies enabled by genomic information and associated techniques. Their
implementation has provided important additional incremental improvements
to the economics of industry-scale manufacturing in which C. glutamicum and its episomal elements are used as a performing host-vector system.