|Gene expression profiling of Corynebacterium glutamicum during anaerobic nitrate respiration: induction of the SOS response for
J. Bacteriol. 193: 1327-1333. 2011.
T. Nishimura, H. Teramoto, M. Inui and H. Yukawa.
The gene expression profile of Corynebacterium glutamicum under anaerobic nitrate respiration revealed marked differences in the
expression levels of a number of genes involved in a variety of cellular
functions, including carbon metabolism and respiratory electron transport
chain, compared to the profile under aerobic conditions using DNA microarrays.
Many SOS genes were upregulated by the shift from aerobic to anaerobic
nitrate respiration. An elongated cell morphology, similar to that induced
by the DivS-mediated suppression of cell division upon cell exposure to
the DNA-damaging reagent mitomycin C, was observed in cells subjected to
anaerobic nitrate respiration. None of these transcriptional and morphological
differences were observed in a recA mutant strain lacking a functional RecA regulator of the SOS response.
The recA mutant cells additionally showed significantly reduced viability compared to wild-type cells similarly grown under anaerobic nitrate respiration. These results suggest a role for the RecA-mediated SOS response in the ability of cells to survive any DNA damage that may result from anaerobic nitrate respiration in C. glutamicum.