|Random genome deletion methods applicable to prokaryotes.
Appl. Microbiol. Biotechnol. 79: 519-526. 2008.
N. Suzuki, M. Inui and H. Yukawa.
Through their enabling of simultaneous identification of multiple non-essential
genes in a genome, large-segment genome deletion methods are an increasingly
popular approach to minimize and tailor microbial genomes for specific
functions. At present, difficulties in identifying target regions for deletion
are a result of inadequate knowledge to define gene essentiality. Furthermore,
with the majority of predicted open reading frames of completely sequenced
genomes still annotated as putative genes, essential or important genes
are found scattered throughout the genomes, limiting the size of non-essential
segments that can be safely deleted in a single sweep. Recently described
large-segment random genome deletion methods that utilize transposons enable
the generation of random deletion strains, analysis of which makes identification
of non-essential genes less tedious. Such and other efforts to determine
the minimum genome content necessary for cell survival continue to accumulate
important information that should help improve our understanding of genome
function and evolution. This review presents an assessment of technological
advancements of random genome deletion methods in prokaryotes to date.