| Abstract |
|---|
|
Living cell reaction process for L-isoleucine and L-valine production. |
|
|
| A new process (Living Cell Reaction Process) for L-isoleucine production using viable, non-growing cells of Brevibacterium flavum AB-07 was optimised using ethanol as the energy souce and alpha-ketobutyric acid (alpha-KB) as precursor.L-valine also could be prodused from glucose at high yield by this process. This process differs from the usual fermentation method in that non-growing cells are used, and the production of L-isoleucine and L-valine were carried out under conditions of repressed cell division and growth. Minimal medium missing the essential growth factor, biotin was employed as the reaction mixture for the production of L-isoleucine and L-valine. The productivity of L-isoleucine and L-valine were 200mmol.1-1.day-1 (molecular yield to alpha-KB: 95%) and 300mmol.1-1.day-1 (molecular yield to glucose: 80%) respectivity. The content of L-isoleucine and L-valine in total amino acids produced in the each mixture were 97% and 96% respectivity. |