|Production of L-malic acid by the cell reusing process.
Process Biochem. 21: 164-166. 1986.
H. Yukawa, H. Yamagata and M. Terasawa.
|Strong fumarase activity was found in Brevibacterium spp. resistant to alpha-amino-n-butyric acid. The production of L-malic
acid from fumaric acid was studies by reusing intact cells. In order to
produce L-malic acid at high yield, the conditions were determined for
the suppression of unwanted by-product (succinic acid) formation.
The production of L-malic acid was carried out reusing intact cells in which the side-reaction was suppressed, and L-malic acid was produced stably from fumaric acid in theoretical yield.