Replacement recombination in Coryneform bacteria: high efficiency integration requirement for non-methylated plasmid DNA.
Biosci. Biotech. Biochem. 57: 2036-2038. 1993.
A.A. Vertès, K. Hatakeyama, M. Inui, M. Kobayashi, Y. Kurusu and H. Yukawa.

We have developed a versatile system that permits efficient manipulation of coryneform bacteria by gene disruption and replacement. Circumventing the methyl-specific restriction system in certain of these bacteria by using non-methylated plasmid DNA was necessary. Integratetive plasmid DNA containing an invitro disrupted copy of the Brevibacterium flavum thrB gene was introduced into B. flavum at an efficiency of 2.4 x 102 integrants/microgram. Integration occurred by homologous recombination either via a Campbell-like mechanism or via a double crossover event. Integrants were stable for over 32 generations.