Abstract

Cloning and nucleotide sequencing of the tyrosine phenol lyase gene from Escherichia intermedia.
Biotech. Lett. 13: 769-772. 1991.
Y. Kurusu, M. Fukushima, K. Kohama, M. Kobayashi, M. Terasawa, H. Kumagai and H. Yukawa.


The tyrosine phenol lyase (TPL) gene was cloned from the genomic DNA of a Escherichia intermedia strain and the nucreotide sequence of the TPL structual gene was determined. The 1801 bp HincII-NruI DNA fragment containing the TPL gene had an open reading frame of 1368 bp and the deduced amino acid sequence was 456 residues long with a molecular weight of 51,441 daltons.